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Genome Sequencing
April 21, 2023 EDT

The Complete Genome Sequences of 19 Species of Snipes (Scolopacidae, Charadriiformes, Aves)

João Marcos G. Capurucho, Taylor Hains, Stacy Pirro, John Bates, Shannon Hackett,
birdgenome
https://doi.org/10.56179/001c.74632
biogenomes
Capurucho, João Marcos G., Taylor Hains, Stacy Pirro, John Bates, and Shannon Hackett. 2023. “The Complete Genome Sequences of 19 Species of Snipes (Scolopacidae, Charadriiformes, Aves).” Biodiversity Genomes, April. https:/​/​doi.org/​10.56179/​001c.74632.
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Abstract

We present the complete genome sequences of 19 species of snipes from 7 genera. Illumina sequencing was performed on genetic material from museum specimens. The reads were assembled using a de novo method followed by a finishing step. The raw and assembled data are publicly available via Genbank.

Introduction

Snipes (Scolopacidae) include 97 extant species (Lepage, Vaidya, and Guralnick 2014; Winkler, Billerman, and Lovette 2020). We present the assembled genomes of 19 species from this family. Tissues were obtained from vouchered museum specimens. More detailed information about the specimen used for each species can be found in the Biosample linked to each genome assembly in Genbank.

Methods

DNA extraction was performed using the Qiagen DNAeasy genomic extraction kit using the standard protocol. A paired-end sequencing library was constructed using the Illumina TruSeq kit according to the manufacturer’s instructions. The library was sequenced on an Illumina Hi-Seq platform in paired-end, 2 × 150 bp format. The resulting fastq files were trimmed of adapter/primer sequence and low-quality regions with Trimmomatic v0.33 (Bolger, Lohse, and Usadel 2014). The trimmed sequence was assembled by SPAdes v2.5 (Bankevich et al. 2012) followed by a finishing step using Zanfona (Kieras, O’Neill, and Pirro 2021).

Results and Data Availability

All data, including raw reads and assembled genome sequence, is available via Genbank.

Bartramia longicauda JAOXNH000000000
Calidris melanotos JAOWAO000000000
Gallinago delicata JAOQGJ000000000
Gallinago gallinago JAOQMJ000000000
Gallinago hardwickii JAOQGE000000000
Gallinago jamesoni JAOQGH000000000
Gallinago media JAOSLN000000000
Gallinago megala JAOQGF000000000
Gallinago nigripennis JAOQGL000000000
Gallinago paraguaiae JAOSZB000000000
Gallinago solitaria JAOQGI000000000
Gallinago stenura JAOQGM000000000
Gallinago undulata JAOTQQ000000000
Limnodromus scolopaceus JAOXNO000000000
Scolopax bukidnonensis JAOWAV000000000
Scolopax minor JAOXNG000000000
Scolopax rusticola JAOWAS000000000
Tringa solitaria JAOXJM000000000
Xenus cinereus JAOXJK000000000

Funding

Funding was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345 Genomic Studies of Eukaryotic Taxa.

Submitted: April 10, 2023 EDT

Accepted: April 21, 2023 EDT

References

Bankevich, Anton, Sergey Nurk, Dmitry Antipov, Alexey A. Gurevich, Mikhail Dvorkin, Alexander S. Kulikov, Valery M. Lesin, et al. 2012. “SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing.” Journal of Computational Biology 19 (5): 455–77. https:/​/​doi.org/​10.1089/​cmb.2012.0021.
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Bolger, Anthony M., Marc Lohse, and Bjoern Usadel. 2014. “Trimmomatic: A Flexible Trimmer for Illumina Sequence Data.” Bioinformatics 30 (15): 2114–20. https:/​/​doi.org/​10.1093/​bioinformatics/​btu170.
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Kieras, M., K. O’Neill, and S. Pirro. 2021. Zanfona, a genome assembly finishing tool for paired-end Illumina reads. https:/​/​github.com/​zanfona734/​zanfona.
Google Scholar
Lepage, Denis, Gaurav Vaidya, and Robert Guralnick. 2014. “Avibase – a Database System for Managing and Organizing Taxonomic Concepts.” Zookeys 420 (June):117–35. https:/​/​doi.org/​10.3897/​zookeys.420.7089.
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Winkler, David W., Shawn M. Billerman, and Irby J. Lovette. 2020. “Sandpipers and Allies (Scolopacidae).” In Birds of the World, edited by Shawn M. Billerman, Brooke K. Keeney, Paul G. Rodewald, and Thomas S. Schulenberg. Cornell Lab of Ornithology. https:/​/​doi.org/​10.2173/​bow.scolop2.01.
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