The Complete Genome Sequence of <i>Chrysina resplendens</i> (Scarabaeidae, Coleoptera) the Golden Jewel Scarab

Douae El Ghoubali; Stacy Pirro; Chakib Nejjari; Hassan Ghazal

doi:10.56179/001c.72798

El Ghoubali, Douae, Stacy Pirro, Chakib Nejjari, and Hassan Ghazal. 2023. “The Complete Genome Sequence of Chrysina Resplendens (Scarabaeidae, Coleoptera) the Golden Jewel Scarab.” Biodiversity Genomes, March. https://doi.org/10.56179/001c.72798.

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Abstract

Chrysina resplendens is a scarab beetle endemic to Central America. We present the whole genome sequence of this species. Illumina sequencing was performed on a genetic sample from a single captive individual. The reads were assembled using a de novo method followed by a series of references from related species for finishing. The raw and assembled data is publicly available via Genbank: Sequence Read Archive (SRR5170512) and Assembly (JAADJY000000000).

Introduction

Chrysina resplendens, or the Golden Jewel Scarab, is found in Central America. It is notable for its shiny metallic gold appearance, prized by insect collectors. This species and others in the genus have evolved an exoskeleton that contains intricate nano-structures that are responsible for its metallic sheen (Fernández del Río, Arwin, and Järrendahl 2016).

Methods

A single wild-caught individual was used for this study. DNA extraction was performed using the Qiagen DNeasy genomic extraction kit using the standard process. A paired-end sequencing library was constructed using the Illumina TruSeq kit according to the manufacturer’s instructions. The library was sequenced on an Illumina Hi-Seq platform in paired-end, 2 × 150bp format. The resulting fastq files were trimmed of adapter/primer sequence and low-quality regions with Trimmomatic v0.33 (Bolger, Lohse, and Usadel 2014). The trimmed sequence was assembled by SPAdes v2.5 (Bankevich et al. 2012) followed by a finishing step using Zanfona (Kieras, O’Neill, and Pirro 2021).

Results

The genome assembly yielded a total sequence length of 68,246,219 bp.

Data availability

Raw and assembled data is publicly available via GenBank:

Raw genome data

https://trace.ncbi.nlm.nih.gov/Traces/sra/?run=SRR5170512

Assembled genome

https://www.ncbi.nlm.nih.gov/nuccore/JAADJY000000000

Funding

Funding was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345 Genomic Studies of Eukaryotic Taxa.

Submitted: February 14, 2023 EDT

Accepted: March 05, 2023 EDT

References

Bankevich, Anton, Sergey Nurk, Dmitry Antipov, Alexey A. Gurevich, Mikhail Dvorkin, Alexander S. Kulikov, Valery M. Lesin, et al. 2012. “SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing.” Journal of Computational Biology 19 (5): 455–77. https://doi.org/10.1089/cmb.2012.0021.

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Bolger, Anthony M., Marc Lohse, and Bjoern Usadel. 2014. “Trimmomatic: A Flexible Trimmer for Illumina Sequence Data.” Bioinformatics 30 (15): 2114–20. https://doi.org/10.1093/bioinformatics/btu170.

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Fernández del Río, Lía, Hans Arwin, and Kenneth Järrendahl. 2016. “Polarizing Properties and Structure of the Cuticle of Scarab Beetles from the Chrysina Genus.” Physical Review E 94 (1): 012409. https://doi.org/10.1103/physreve.94.012409.

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Kieras, M., K. O’Neill, and S. Pirro. 2021. Zanfona, a genome assembly finishing tool for paired-end Illumina reads. https://github.com/zanfona734/zanfona.

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The Complete Genome Sequence of Chrysina resplendens (Scarabaeidae, Coleoptera) the Golden Jewel Scarab