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Genome Sequencing
March 04, 2023 EDT

The Complete Genome Sequences of 13 Species of Brachycnemina (Cnidaria, Hexacorallia, Anthozoa, Zoantharia)

Maria E. A. Santos, Hiroki Kise, Chloé Julie Loïs Fourreau, Angelo Poliseno, Stacy Pirro, James Davis Reimer,
BrachycneminagenomeZoantharia
https://doi.org/10.56179/001c.72778
biogenomes
Santos, Maria E. A., Hiroki Kise, Chloé Julie Loïs Fourreau, Angelo Poliseno, Stacy Pirro, and James Davis Reimer. 2023. “The Complete Genome Sequences of 13 Species of Brachycnemina (Cnidaria, Hexacorallia, Anthozoa, Zoantharia).” Biodiversity Genomes, March. https:/​/​doi.org/​10.56179/​001c.72778.
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Abstract

We present the complete genome sequences of 13 species of the zoantharian suborder Brachycnemina (Haddon, and Shackleton, 1891; Poliseno et al., 2020). Genome sequencing was performed on genetic material from single wild-collected individuals. For each species the sequence reads were assembled using a de novo method followed by a finishing step. The raw and assembled data are publicly available via GenBank.

Introduction

We present the assembled draft genome sequences from 13 extant species of the zoantharian suborder Brachycnemina. Detailed information about the specimen used for each species can be found in the Biosample linked to each genome assembly in GenBank.

Methods

DNA extraction was performed using the Qiagen DNAeasy genomic extraction kit using the standard process. A paired-end sequencing library was constructed using the Illumina TruSeq kit according to the manufacturer’s instructions. The libraries were sequenced on an Illumina HiSeq platform in paired-end (2 × 150 bp) format. The resulting fastq files were trimmed of adapter/primer sequences and low-quality regions with Trimmomatic v0.33 (Bolger, Lohse, and Usadel 2014). The trimmed sequences were assembled by SPAdes v2.5 (Bankevich et al. 2012) followed by a finishing step using Zanfona (Kieras, O’Neill, and Pirro 2021).

Results and Data Availability

All data, including raw reads and assembled genome sequence, are available via GenBank.

Isaurus tuberculatus JAOEHV000000000
Palythoa caribaeorum JAOCNB000000000
Palythoa grandiflora JAOCPT000000000
Palythoa grandis JAOEJH000000000
Palythoa heliodiscus JAOBYJ000000000
Palythoa mutuki JAODFW000000000
Sphenopus marsupialis JAOBRF000000000
Zoanthus gigantus JAOBBB000000000
Zoanthus pulchellus JAOBRB000000000
Zoanthus sanctaehelenae JAOTRI000000000
Zoanthus sansibaricus JAPEIX000000000
Zoanthus sociatus JAOBAY000000000
Zoanthus solanderi JANCMA000000000

Funding

Funding was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345 Genomic Studies of Eukaryotic Taxa.

Submitted: February 22, 2023 EDT

Accepted: March 04, 2023 EDT

References

Bankevich, Anton, Sergey Nurk, Dmitry Antipov, Alexey A. Gurevich, Mikhail Dvorkin, Alexander S. Kulikov, Valery M. Lesin, et al. 2012. “SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing.” Journal of Computational Biology 19 (5): 455–77. https:/​/​doi.org/​10.1089/​cmb.2012.0021.
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Bolger, Anthony M., Marc Lohse, and Bjoern Usadel. 2014. “Trimmomatic: A Flexible Trimmer for Illumina Sequence Data.” Bioinformatics 30 (15): 2114–20. https:/​/​doi.org/​10.1093/​bioinformatics/​btu170.
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Haddon, A.C., and A.M. Shackleton. 1891. “Actiniae: I. Zoantheae.” In Reports on the Zoological Collections Made in the Torres Straits by Professor A.C. Haddon, 1888–1889, 4:673–658. Scientific Transations of the Royal Dublin Society.
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Kieras, M., K. O’Neill, and S. Pirro. 2021. Zanfona, a genome assembly finishing tool for paired-end Illumina reads. https:/​/​github.com/​zanfona734/​zanfona.
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Poliseno, Angelo, Maria Eduarda Alves Santos, Hiroki Kise, Brooks Macdonald, Andrea M. Quattrini, Catherine S. McFadden, and James Davis Reimer. 2020. “Evolutionary Implications of Analyses of Complete Mitochondrial Genomes across Order Zoantharia (Cnidaria: Hexacorallia).” Journal of Zoological Systematics and Evolutionary Research 58 (4): 858–68. https:/​/​doi.org/​10.1111/​jzs.12380.
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