Methods

Tissues from single, wild-collected individuals were used for this study. DNA extraction was performed using the Qiagen DNEasy genomic extraction kit using the standard process. Paired-end sequencing libraries were constructed using the Illumina TruSeq kit according to the manufacturer’s instructions. The libraries were sequenced on an Illumina Hi-Seq platform in paired-end, 2 × 150bp format. The resulting fastq files were trimmed of adapter/primer sequences and low-quality regions with Trimmomatic v0.33 (Bolger et al. 2014). The trimmed sequence was assembled by SPAdes v3.15.4 (Bankevich et al. 2012) followed by a finishing step using Zanfona (Kieras et al. 2021).

Results and Data Availability

All raw data and assembled genomes are available via Genbank.

taxname specimen_voucher accession
Asbestopluma belgicae USNM 43495 SRR39277383
Asbestopluma ramosa USNM 34704 SRR39288786
Axoniderma corona USNM 1478697 SRR39297573
Chondrocladia clavata USNM 43601 SRR39288787
Chondrocladia grandis USNM 1558251 SRR39277469
Chondrocladia lyra USNM 1424173 SRR39277382
Chondrocladia verticillata USNM 1482939 SRR39277468
Chondrocladia virgata USNM 1234806 SRR39277575
Cladorhiza bathycrinoides USNM 1695236 SRR39277605
Guitarra abbotti USNM 1478705 SRR39277576
Guitarra fimbriata USNM 1478707 SRR39277380
Lycopodina subtile USNM 1424165 SRR39277481
Mycale microsigmatosa USNM 1660175 SRR39268251
Mycale tylota USNM 1478734 SRR39277589

Funding

Funding was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345 Genomic Studies of Eukaryotic Taxa.