Methods

Tissues from single, wild-collected individuals were used for this study. DNA extraction was performed using the Qiagen DNEasy genomic extraction kit using the standard process. Paired-end sequencing libraries were constructed using the Illumina TruSeq kit according to the manufacturer’s instructions. The libraries were sequenced on an Illumina Hi-Seq platform in paired-end, 2 × 150bp format. The resulting fastq files were trimmed of adapter/primer sequences and low-quality regions with Trimmomatic v0.33 (Bolger et al. 2014). The trimmed sequence was assembled by SPAdes v3.15.4 (Bankevich et al. 2012) followed by a finishing step using Zanfona (Kieras et al. 2021).

Results and Data Availability

All raw data and assembled genomes are available via Genbank.

taxname accession specimen_voucher
Boiga cyanea JBYEHR000000000 NRB 0617
Bungarus candidus JBYVEH000000000 NRB 0590
Calloselasma rhodostoma JBYIYH000000000 NRB 0592
Cyrtodactylus intermedius JBYVEO000000000 NRB 0540
Dendrelaphis pictus JBYIXT000000000 NRB 0621
Duttaphrynus melanostictus JBXGFQ000000000 NRB 0531
Fejervarya limnocharis JBYOGR000000000 NRB 0548
Gekko gecko JBYIYK000000000 NRB 0552
Hemidactylus frenatus JBYIXY000000000 NRB 0554
Hemidactylus platyurus JBZFXA000000000 NRB 0556
Homalopsis mereljcoxi JBYVER000000000 NRB 0611
Kalophrynus interlineatus JBYIYB000000000 NRB 0562
Kaloula pulchra JBXGFO000000000 NRB 0565
Lycodon davisonii JBYOHB000000000 NRB 0619
Naja fuxi JBYOHI000000000 NAFU-KH
Naja kaouthia JBYOHD000000000 NRB 0509
Naja siamensis JBYOGX000000000 NRB 0510
Pareas berdmorei JBWWBL000000000 NRB 0594
Physignathus cocincinus JBYOHF000000000 NRB 0537
Sphenomorphus maculatus JBYOGZ000000000 NRB 0525
Trimeresurus cardamomensis JBWWCN000000000 NRB 0553
Trimeresurus macrops JBWWCO000000000 NRB 0521

Funding

Funding was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345 Genomic Studies of Eukaryotic Taxa.