Methods

Tissues from single, wild-collected individuals from Ethiopia, Guyana, Mexico and the United States were used for this study. DNA extraction was performed using the Qiagen DNEasy genomic extraction kit using the standard process. Paired-end sequencing libraries were constructed using the Illumina TruSeq kit according to the manufacturer’s instructions. The libraries were sequenced on an Illumina Hi-Seq platform in paired-end, 2 × 150bp format. The resulting fastq files were trimmed of adapter/primer sequences and low-quality regions with Trimmomatic v0.33 (Bolger et al. 2014). The trimmed sequence was assembled by SPAdes v3.15.4 (Bankevich et al. 2012) followed by a finishing step using Zanfona (Kieras et al. 2021).

Results and Data Availability

All raw data and assembled genomes are available via Genbank.

taxname specimen_voucher accession
Agama hispida UPRMGENRESCOL_1923_TJC1510 JBWYGI000000000
Agama spinosa UPRMGENRESCOL_1754_TJC1370 JBWYGW000000000
Bothrops brazili UPRMGENRESCOL_2031_TJC1619 JBYHFE000000000
Broadleysaurus major UPRMGENRESCOL_1776_TJC1392 JBYVEF000000000
Chironius fuscus UPRMGENRESCOL_2016_TJC1604 JBYVEK000000000
Chlorosoma viridissimum UPRMGENRESCOL_2037_TJC1625 JBYVEI000000000
Cnemidophorus lemniscatus UPRMGENRESCOL_1924_TJC1511 JBYIXR000000000
Corallus hortulana UPRMGENRESCOL_1980_TJC1567 JBYVEJ000000000
Dipsas pavonina UPRMGENRESCOL_2033_TJC1621 JBYVEM000000000
Echis pyramidum UPRMGENRESCOL_1868_TJC1484 JBYVEL000000000
Erythrolamprus typhlus UPRMGENRESCOL_2017_TJC1605 JBYIXS000000000
Heliobolus spekii UPRMGENRESCOL_1791_TJC1407 JBYIXX000000000
Hemidactylus angulatus UPRMGENRESCOL_1832_TJC1448 JBYIYA000000000
Hemidactylus awashensis UPRMGENRESCOL_1768_TJC1384 JBYIXW000000000
Hemidactylus isolepis UPRMGENRESCOL_1797_TJC1413 JBYIXV000000000
Hemidactylus jubensis UPRMGENRESCOL_1837_TJC1453 JBYIYL000000000
Hemidactylus mabouia UPRMGENRESCOL_1927_TJC1514 JBZFWZ000000000
Hemidactylus palaichthus UPRMGENRESCOL_1914_TJC1501 JBYIXZ000000000
Hemidactylus robustus UPRMGENRESCOL_1801_TJC1417 JBYQWJ000000000
Lachesis muta UPRMGENRESCOL_1994_TJC1582 JBYOGU000000000
Lampropeltis nigra UPRMGENRESCOL_593_TJC119 JBYVEQ000000000
Latastia longicaudata UPRMGENRESCOL_1792_TJC1408 JBXGFK000000000
Leptodeira annulata UPRMGENRESCOL_1935_TJC1522 JBYOGS000000000
Lygodactylus keniensis UPRMGENRESCOL_1834_TJC1450 JBUZWP000000000
Lygodactylus somalicus UPRMGENRESCOL_1806_TJC1422 JBZUHU000000000
Masticophis flagellum UPRMGENRESCOL_591_TJC117 JBYQWE000000000
Nerodia erythrogaster UPRMGENRESCOL_524_TJC25 JBYOGO000000000
Nerodia sipedon UPRMGENRESCOL_616_TJC142 JBYOGW000000000
Oxybelis aeneus UPRMGENRESCOL_1967_TJC1554 JBYOHE000000000
Oxyrhopus occipitalis UPRMGENRESCOL_2032_TJC1620 JBZFWU000000000
Oxyrhopus petolarius UPRMGENRESCOL_1671_TJC1296 JBWWBJ000000000
Pantherophis emoryi UPRMGENRESCOL_614_TJC140 JBWWBK000000000
Pristurus rupestris UPRMGENRESCOL_1786_TJC1402 JBWWBP000000000
Pseudoboa neuwiedii UPRMGENRESCOL_1919_TJC1506 JBWWBQ000000000
Rhamphiophis rostratus UPRMGENRESCOL_1784_TJC1400 JBWWAZ000000000
Storeria dekayi UPRMGENRESCOL_589_TJC115 JBWSOL000000000
Trachylepis quinquetaeniata UPRMGENRESCOL_1756_TJC1372 JBWWCL000000000
Trachylepis varia UPRMGENRESCOL_1793_TJC1409 JBWWCM000000000
Xenagama wilmsi UPRMGENRESCOL_1762_TJC1378 JBXGFD000000000

Funding

Funding was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345 Genomic Studies of Eukaryotic Taxa and by NSF grant DBI2334779 to TJC.

Acknowledgements

TJC thanks the Ethiopian Wildlife Conservation Authority, The Guyanese Environmental Protection Agency, SEMARNAT Mexico and the Florida FWC for research, collection and export permits pertaining to fieldwork associated with these specimens.