Methods
Tissues from single, wild-collected individuals were used for this study. DNA extraction was performed using the Qiagen DNEasy genomic extraction kit using the standard process. Paired-end sequencing libraries were constructed using the Illumina TruSeq kit according to the manufacturer’s instructions. The libraries were sequenced on an Illumina Hi-Seq platform in paired-end, 2 × 150bp format. The resulting fastq files were trimmed of adapter/primer sequences and low-quality regions with Trimmomatic v0.33 (Bolger et al, 2014). The trimmed sequence was assembled by SPAdes v3.15.4 (Bankevich et al, 2012) followed by a finishing step using Zanfona (Kieras et al., 2021).
Results and Data Availability
All raw data and assembled genomes are available via Genbank.
Funding
Funding was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345 Genomic Studies of Eukaryotic Taxa. This work was supported in part by The Negaunee Foundation fund for science at The Field Museum.
Acknowledgements
Special thanks to Drs Katuala Pionus, Casimir Nebesse Mololo and Dieudonne Upoki Agenong’a at the Faculty of Science, University of Kisangani for providing logistical assistance to our team prior and during the course of our survey work.