Methods

Tissues from single, wild-collected individuals were used for this study. DNA extraction was performed using the Qiagen DNEasy genomic extraction kit using the manufacturer’s protocol. Paired-end sequencing libraries were constructed using the Illumina TruSeq kit according to the manufacturer’s protocol. The libraries were sequenced on an Illumina Hi-Seq platform in paired-end, 2 × 150bp format. The resulting fastq files were trimmed of adapter/primer sequences and low-quality regions with Trimmomatic v0.33 (Bolger et al. 2014). The trimmed sequence was assembled by SPAdes v3.15.4 (Bankevich et al. 2012) followed by a finishing step using Zanfona (Kieras et al. 2021).

Results and Data Availability

Species Ploidy Accession Specimen ID Sex
Xenopus borealis 4n SRR35968864 Evans_17C3 F
Xenopus boumbaensis 8N SRR35972782 AMNH17285 M
Xenopus fischbergi 4n SRR35973704 Evans_18DF F
Xenopus fraseri 4n SRR35964371 BJE4204 F
Xenopus itombwensis 8n SRR38286237 BJE3376 M
Xenopus lenduensis 8n SRR38285470 Evans_lendu_20 M
Xenopus longipes 12n SRR38285167 Evans_male101 M
Xenopus muelleri 4n SRR35972780 Evans_17B3 F
Xenopus parafraseri 4n SRR35972781 BJE3065 F
Xenopus pygmaeus 4n SRR35964928 Evans_15 F
Xenopus wittei 8n SRR38285187 BJE4311 F

Funding

Funding was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345 Genomic Studies of Eukaryotic Taxa.