Methods
Specimen 1302, a female, was collected from the Laguna Mountains population (33.33028, -116.83775) on 12 May 2013 and was maintained in captivity to establish a captive rearing program conducted through The Endangered Butterfly Project at The Teaching Zoo at Moorpark College (Longcore et al. 2014). This work was conducted in accordance with the U.S. Fish and Wildlife Service Carlsbad Fish and Wildlife Office’s subpermit, LOS-3761. Geographical and collection data were recorded for the specimen. Husbandry and captive rearing were housed at The Teaching Zoo at Moorpark College.
DNA extraction was performed using the Qiagen DNEasy genomic extraction kit using the standard process. Paired-end sequencing libraries were constructed using the Illumina TruSeq kit according to the manufacturer’s instructions. The libraries were sequenced on an Illumina Hi-Seq platform in paired-end, 2 × 150bp format. The resulting fastq files were trimmed of adapter/primer sequences and low-quality regions with Trimmomatic v0.33 (Bolger et al. 2014). The trimmed sequence was assembled by SPAdes v3.15.4 (Bankevich et al. 2012) followed by a finishing step using Zanfona (Kieras et al. 2021).
Data availability
Acknowledgements
The authors are grateful to U.S. Fish and Wildlife Service, California Department of Fish and Wildlife, The Urban Wildlands Group, Osborne Biological Consulting, The Teaching Zoo, Moorpark College, San Diego Zoo, and Iridian Genomes for funding, permitting, housing, and fieldwork that made the captive rearing program and subsequent genomic analysis possible.
Funding
Funding for sequencing was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345 Genomic Studies of Eukaryotic Taxa.