Methods
Specimen 1703, a female Apodemia mormo langei, was collected from the Antioch Dunes population during the 2017 flight season at 10:02 AM (38.01348, -121.78278) and was maintained in captivity as part of conservation breeding efforts conducted through The Endangered Butterfly Project at The Teaching Zoo at Moorpark College (Johnson et al. 2017). This work was conducted in accordance with applicable federal and state permits # FWSADNWR-1. Letter of Authorization from Fish and Wildlife LOA-06.
DNA extraction was performed using the Qiagen DNEasy genomic extraction kit using the standard process. Paired-end sequencing libraries were constructed using the Illumina TruSeq kit according to the manufacturer’s instructions. The libraries were sequenced on an Illumina Hi-Seq platform in paired-end, 2 × 150bp format. The resulting fastq files were trimmed of adapter/primer sequences and low-quality regions with Trimmomatic v0.33 (Bolger et al. 2014). The trimmed sequence was assembled by SPAdes v3.15.4 (Bankevich et al. 2012) followed by a finishing step using Zanfona (Kieras et al. 2021).
Data availability
Acknowledgements
The authors are grateful to the U.S. Fish and Wildlife Service (USFWS), California Department of Fish and Wildlife (CDFW), The Urban Wildlands Group, The Teaching Zoo at Moorpark College, Moorpark College, Osborne Consulting, and Iridian Genomes for funding, permitting, housing, husbandry, and field support that made both the captive rearing program and subsequent genomic sampling possible.
Funding
Funding was provided by Iridian Genomes through grant IRGEN_RG_2021-1345, Genomic Studies of Eukaryotic Taxa.