Methods

Tissues from single, wild-collected individuals were used for this study. DNA extraction was performed using the Qiagen DNEasy genomic extraction kit using the standard process. Paired-end sequencing libraries were constructed using the Illumina TruSeq kit according to the manufacturer’s instructions. The libraries were sequenced on an Illumina Hi-Seq platform in paired-end, 2 × 150bp format. The resulting fastq files were trimmed of adapter/primer sequences and low-quality regions with Trimmomatic v0.33 (Bolger et al. 2014). The trimmed sequence was assembled by SPAdes v3.15.4 (Bankevich et al. 2012) followed by a finishing step using Zanfona (Kieras et al. 2021).

Results and Data Availability

taxid species specimen_voucher bioproject
8499 Caiman crocodilus UPRMGENRESCOL_4184_TJC1737 JBTXPI000000000
103699 Chlamydosaurus kingii UPRMGENRESCOL_4185_TJC1738 JBTVYJ000000000
88867 Gymnophthalmus underwoodi UPRMGENRESCOL_3211_TJC1727 JBTVYH000000000
1496311 Malayopython reticulatus UPRMGENRESCOL_4148_TJC1732 JBTXPH000000000
176946 Python bivittatus UPRMGENRESCOL_4186_TJC1733 JBTVYI000000000

Funding

Funding was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345 Genomic Studies of Eukaryotic Taxa. TJC was supported by NSF grants DBI#2334779, DEB#2406685.

Acknowledgements

We thank the Puerto Rican DRNA and Proyectos Retic with assistance in donating specimens.