Methods
An individual seedling derived from in vitro culture of each species was used to extract genomic DNA using a commercially available kit (DNeasy Plant Mini kit, Qiagen) following the manufacturer’s protocol. Paired-end sequencing libraries were prepared with the Illumina TruSeq kit according to the manufacturer’s instructions and subsequently sequenced on an Illumina NovaSeq 6000 platform in paired-end mode (format 2×150 bp). During assembly, the resulting FASTQ files were uploaded to the Huitzilin high-performance computing cluster (INECOL), where adapter and primer sequences, as well as low-quality regions, were removed using fastp v0.23.4 (Chen et al. 2018). High-quality paired-end reads were then assembled with SPAdes v3.13.0 (Bankevich et al. 2012).
Results and Data Availability
Funding
Funding was provided by Iridian Genomes, grant IRGEN_RG_2021-1345 Genomic Studies of Eukaryotic Taxa, and by the 20270 Fiscal Project (CICY) for sampling in the field.
Acknowledgements
The authors would like to thank Wilbert Puc for allowing us to collect C. palmata seeds on his field parcel and Élide Aviles-Berzunza for her help during field sampling.