Methods

Tissues from single, wild-collected individuals were used for this study. DNA extraction was performed using the Qiagen DNEasy genomic extraction kit using the standard process. Paired-end sequencing libraries were constructed using the Illumina TruSeq kit according to the manufacturer’s instructions. The libraries were sequenced on an Illumina Hi-Seq platform in paired-end, 2 × 150bp format. The resulting fastq files were trimmed of adapter/primer sequences and low-quality regions with Trimmomatic v0.33 (Bolger et al. 2014). The trimmed sequence was assembled by SPAdes v3.15.4 (Bankevich et al. 2012) followed by a finishing step using Zanfona (Kieras et al. 2021).

Results and Data Availability

taxname accession specimen_voucher country
Anastomus lamelligerus JBFODX000000000 FMNH 192043 Somalia
Ephippiorhynchus senegalensis JBFPEW000000000 FMNH 85840 Swaziland
Leptoptilos crumeniferus JBFSEP000000000 FMNH 269478 Cameroon
Mycteria ibis JBKOKA000000000 FMNH 192046 Somalia

Funding

Funding was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345 Genomic Studies of Eukaryotic Taxa.