The complete genome sequences of 73 species of Anseriformes (Aves, Chordata)

Methods

Tissues from single, wild-collected individuals were used for this study. DNA extraction was performed using the Qiagen DNEasy genomic extraction kit using the standard process. Paired-end sequencing libraries were constructed using the Illumina TruSeq kit according to the manufacturer’s instructions. The libraries were sequenced on an Illumina Hi-Seq platform in paired-end, 2 × 150bp format. The resulting fastq files were trimmed of adapter/primer sequences and low-quality regions with Trimmomatic v0.33 (Bolger et al. 2014). The trimmed sequence was assembled by SPAdes v3.15.4 (Bankevich et al. 2012) followed by a finishing step using Zanfona (Kieras et al. 2021). We would like to thank the following museums for contributing loans to this project: the Academy of Natural Sciences of Drexel University, Bell Museum of Natural History, Burke Museum of Natural History and Culture, Field Museum of Natural History, Florida Museum of Natural History, Natural History Museum of Denmark, University of Alaska Museum of the North, and the University of Kansas Natural History Museum.

Results and Data Availability

Funding

Funding was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345 Genomic Studies of Eukaryotic Taxa.