Methods

Single wild-collected individuals were sampled for this study. Details of sample collection, permitting, and localities are given by Pyron et al. (2013). DNA extraction was performed using the Qiagen DNAeasy genomic extraction kit using the standard protocol. A paired-end sequencing library was constructed using the Illumina TruSeq kit according to the manufacturer’s instructions. The library was sequenced on an Illumina Hi-Seq platform in paired-end, 2 × 150 bp format. The resulting fastq files were trimmed of adapter/primer sequence and low-quality regions with Trimmomatic v0.33 (Bolger, Lohse, and Usadel 2014). The trimmed sequence was assembled by SPAdes v2.5 (Bankevich et al. 2012) followed by removal of contaminants using NCBI Foreign Contamination Screen (FCS; Astashyn et al. 2024). A finishing step was performed using Zanfona (Kieras, O’Neill, and Pirro 2021).

Results and Data Availability

All data, including raw reads and assembled genome sequences, are available via Genbank.

species isolate biosample genome
Colubridae
Ahaetulla nasuta RAP0458 SAMN44529004 JBJNRS000000000
Ahaetulla pulverulenta* RS-C SAMN44529021 JBJPGR000000000
Amphiesma stolatum RS-G SAMN44529017 JBJJOR000000000
Aspidura guentheri* RAP0437 SAMN44529000 JBKOJE000000000
Boiga barnesii* RAP0499 SAMN44529010 JBKIYP000000000
Boiga ceylonensis RAP0515 SAMN44529013 JBLLKT000000000
Boiga ranawanei*1 RAP05431 SAMN44529016 JBLLKS000000000
Boiga trigonata RAP0478 SAMN44529005 JBJPGO000000000
Dendrelaphis bifrenalis* RS-E SAMN44529018 JBJPGS000000000
Dendrelaphis caudolineolatus RS-W SAMN44529019 JBJPHK000000000
Dendrelaphis schokari* RAP0494 SAMN44529009 JBJPGU000000000
Dendrelaphis tristis RAP0526 SAMN44529015 JBKOPV000000000
Fowlea asperrima* RAP0430 SAMN44528999 JBJNRT000000000
Fowlea unicolor2 RAP0482 SAMN44529006 JBJNRV000000000
Lycodon aulicus RAP0440 SAMN44529001 JBJPGQ000000000
Lycodon carinatus* RAP0447 SAMN44529003 JBLLKQ000000000
Oligodon sublineatus* RS-141 SAMN44529024 JBJPGW000000000
Oligodon taeniolatus RS-136 SAMN44529022 JBJPGT000000000
Sibynophis subpunctatus RAP0491 SAMN44529008 JBKKFB000000000
Elapidae
Bungarus caeruleus RAP0514 SAMN44529012 JBJJOK000000000
Bungarus ceylonicus* RAP0441 SAMN44529002 JBJPGP000000000
Hydrophis schistosus RAP0487 SAMN44529007 JBJJOL000000000
Uropeltidae
Rhinophis saffragamus* RS-140 SAMN44529023 JBKOJP000000000
Viperidae
Daboia russelii RAP0501 SAMN44529011 JBJJOO000000000
Hypnale hypnale RS-139 SAMN44529020 JBJJOM000000000
Peltopelor trigonocephalus*3 RAP0519 SAMN44529014 JBLLKO000000000

* Endemic species
1 Previously cited as Boiga beddomei
2 Previously cited as Fowlea piscator
3 Previously cited as Trimeresurus trigonocephalus

Funding

Funding was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345 Genomic Studies of Eukaryotic Taxa.