Loading [Contrib]/a11y/accessibility-menu.js
Skip to main content
biogenomes
  • Menu
  • Articles
    • Genome Sequencing
    • All
  • For Authors
  • Editorial Board
  • About
  • Open Access
  • Peer Review
  • search

RSS Feed

Enter the URL below into your favorite RSS reader.

http://localhost:52307/feed
Genome Sequencing
March 27, 2025 EDT

The Complete Genome Sequence of the Caspian seal Pusa caspica (Gmelin, 1788)

André Gomes-dos-Santos, Elsa Froufe, Viatcheslav V. Rozhnov, Ivan Bolotov, Ilya G. Meschersky, Sergey I. Meschersky, Maria A. Solovyeva, Fedor V. Klimov, L. Filipe C. Castro, Manuel Lopes-Lima,
https://doi.org/10.56179/001c.133591
biogenomes
Gomes-dos-Santos, André, Elsa Froufe, Viatcheslav V. Rozhnov, Ivan Bolotov, Ilya G. Meschersky, Sergey I. Meschersky, Maria A. Solovyeva, Fedor V. Klimov, L. Filipe C. Castro, and Manuel Lopes-Lima. 2025. “The Complete Genome Sequence of the Caspian Seal Pusa Caspica (Gmelin, 1788).” Biodiversity Genomes, March. https:/​/​doi.org/​10.56179/​001c.133591.
Save article as...▾
Download all (1)
  • Download

Sorry, something went wrong. Please try again.

If this problem reoccurs, please contact Scholastica Support

Error message:

undefined

View more stats

Abstract

The Caspian seal (Pusa caspica) is a member of the earless (Phocidae) family, and the only mammal endemic to the brackish Caspian Sea. We generated a whole genome sequence of this species using the Illumina Paired-end short reads whole genome sequencing approach. The DNA reads were assembled using the Abyss2 de novo assembly approach. The raw data and genome assembly were deposited in NCBI, being publicly available via GenBank, with Sequence Read Archive SRR32005400, assembled genome Accession (JBLQUN010000000), under BioProject PRJNA1211000.

Introduction

The Caspian seal (Pusa caspica), first described by Gmelin in 1788, is the only endemic mammal of the Caspian Sea, where it thrives in brackish conditions (Ranjbar Jafarabadi et al. 2021). Alongside the Baikal seal (Pusa sibirica), it is one of only two completely landlocked species in the Phocidae family. However, the Caspian seal faces mounting threats from climate change, natural processes occurring in the Caspian Sea, habitat degradation and pollution (Rozhnov et al. 2022; S. Goodman and Dmitrieva 2016; Ranjbar Jafarabadi et al. 2021). Over the past century, its population has declined by more than 70%. In recent years, the number of seals has remained at a relatively stable level of about 302 thousand individuals (Sidorov et al. 2023). As a result, the species is currently classified as Endangered (EN) on the IUCN Red List of Threatened Species (S. Goodman and Dmitrieva 2016).

Although Caspian seals are considered a single population distributed throughout the Caspian Sea, genetic data on the species remains scarce (Arnason et al. 2006; Palo and Väinölä 2006), and no comprehensive population genetics study has been conducted to date. The Caspian Sea represents one of the most extreme environments inhabited by any pinniped, with air temperatures ranging from −35°C in winter to +40°C in summer (S. J. Goodman 2018). Water depths vary dramatically, from less than 1 meter in large river deltas to 1,000 meters in the central and southern basins (S. J. Goodman 2018). These harsh conditions have driven several evolutionary adaptations in Caspian seals, including distinct phenotypic traits such as large eyes, robust masticatory muscles, and a unique tooth structure (Endo et al. 2002; S. J. Goodman 2018).

Photo by D. Glazov (IEE RAS).

Methods

A muscle tissue sample was obtained from the naturally injured forelimb of a wild Pusa caspica specimen and preserved in 96% ethanol. The sample was collected during a veterinary procedure in which a torn muscle fragment was surgically removed as part of the animal’s treatment. This specimen was sampled in the waters of the Republic of Kazakhstan in November 2020 during a scientific campaign conducted within the framework of the Russia-Kazakhstan “Program for the Study of the Caspian Seal of the Northern Caspian (2019-2023)”. Total DNA was then extracted using the Qiagen MagAttract HMW DNA extraction kit (Dobra). Whole genome sequencing was performed using a Truseq Nano DNA Illumina library preparation kit, followed by paired-end sequencing (2 × 150 bp) on an Illumina NovaSeq6000 machine, at Macrogen Inc. (Korea). Raw sequencing reads were trimmed of adapters and quality filtered using Trimmomatic v.0.38 (Bolger, Lohse, and Usadel 2014), and read quality was validated using fastqc before and after trimming. The genome assembly was generated with Abyss2 (Jackman et al. 2017) using the paired and unpaired trimmed reads and specifying a 96 k-mer length.

Results

The genome assembly yielded a total sequence length of 2.36 Gbp over 442 199 scaffolds and a scaffold N50 length of 48 263bp.

Data availability

Raw and assembled data is publicly available via GenBank:

Raw genome data
https://trace.ncbi.nlm.nih.gov/Traces/?view=run_browser&acc=SRR32005400

Assembled genome:
https://www.ncbi.nlm.nih.gov/nuccore/JBLQUN010000000


Funding

The collection of material for the study was carried out within the framework of the Russia-Kazakhstan “Program for the Study of the Caspian Seal of the Northern Caspian (2019-2023)” with financial support “Kazakhstan Agency of Applied Ecology” LLP and North Caspian Operating Company N.V. This research was funded by national funds through FCT – Fundação para a Ciência e a Tecnologia within the scope of the Strategic Funding UIDB/04423/2020 (https://doi.org/10.54499/UIDB/04423/2020), UIDP/04423/2020 (https://doi.org/10.54499/UIDP/04423/2020), and LA/P/0101/2020 (https://doi.org/10.54499/LA/P/0101/2020). Fundação para a Ciência e a Tecnologia also supported AGS (2023.07625.CEECIND), EF (CEECINST/00027/2021/CP2789/CT0003 and DOI identifier https://doi.org/10.54499/CEECINST/00027/2021/CP2789/CT0003) and MLL (CEECINSTLA/00020/2022).

Submitted: March 19, 2025 EDT

Accepted: March 27, 2025 EDT

References

Arnason, U., A. Gullberg, A. Janke, M. Kullberg, N. Lehman, E. A. Petrov, and R. Väinölä. 2006. “Pinniped Phylogeny and a New Hypothesis for Their Origin and Dispersal.” Molecular Phylogenetics and Evolution 41 (2): 345–54. https:/​/​doi.org/​10.1016/​j.ympev.2006.05.022.
Google Scholar
Bolger, A. M., M. Lohse, and B. Usadel. 2014. “Trimmomatic: A Flexible Trimmer for Illumina Sequence Data.” Bioinformatics 30 (15): 2114–20. https:/​/​doi.org/​10.1093/​bioinformatics/​btu170.
Google Scholar
Endo, H., S. Sakata, T. Arai, and N. Miyazaki. 2002. “The Muscles of Mastication in the Caspian Seal (Phoca Caspica).” Anatomia, Histologia, Embryologia 31 (5): 262–65. https:/​/​doi.org/​10.1046/​j.1439-0264.2002.00372.x.
Google Scholar
Goodman, S., and L. Dmitrieva. 2016. “Pusa Caspica.” The IUCN Red List of Threatened Species 2016.
Google Scholar
Goodman, S. J. 2018. “Caspian Seal.” In Encyclopedia of Marine Mammals, 164–66. Elsevier. https:/​/​doi.org/​10.1016/​B978-0-12-804327-1.00085-6.
Google Scholar
Jackman, S. D., B. P. Vandervalk, H. Mohamadi, J. Chu, S. Yeo, S. A. Hammond, G. Jahesh, et al. 2017. “ABySS 2.0: Resource-Efficient Assembly of Large Genomes Using a Bloom Filter.” Genome Research 27 (5): 768–77. https:/​/​doi.org/​10.1101/​gr.214346.116.
Google Scholar
Palo, J. U., and R. Väinölä. 2006. “The Enigma of the Landlocked Baikal and Caspian Seals Addressed through Phylogeny of Phocine Mitochondrial Sequences.” Biological Journal of the Linnean Society 88 (1): 61–72. https:/​/​doi.org/​10.1111/​j.10958312.2006.00607.x.
Google Scholar
Ranjbar Jafarabadi, A., S. Mashjoor, S. Mohamadjafari Dehkordi, A. Riyahi Bakhtiari, and T. Cappello. 2021. “Emerging POPs-Type Cocktail Signatures in Pusa Caspica in Quantitative Structure-Activity Relationship of Caspian Sea.” Journal of Hazardous Materials 406:124334. https:/​/​doi.org/​10.1016/​j.jhazmat.2020.124334.
Google Scholar
Rozhnov, V. V., V. A. Bizikov, M.-R. D. Magomedov, M. A. Solovyeva, S. V. Shipulin, V. V. Kuznetsov, I. V. Suvorova, L. K. Sidorov, I. F. Belokobylsky, and V. V. Proskurina. 2022. “Death of the Caspian Seals on the Dagestan Coast of the Caspian Sea in the Autumn of 2020 and Its Possible Reasons.” Trudy VNIRO 187:87–109. https:/​/​doi.org/​10.36038/​2307-3497-2022-187-87-109.
Google Scholar
Sidorov, L. K., V. A. Bizikov, V. V. Rozhnov, S. V. Shipulin, V. V. Kuznetsov, I. F. Belokobylsky, N. A. Myagkiy, M. A. Solovyeva, D. M. Glazov, and E. A. Nazarenko. 2023. “Abundance and Distribution of the Caspian Seals on Ice in the Russian Part of the Northern Caspian in February-March 2023.” Trudy VNIRO 193:82–100. https:/​/​doi.org/​10.36038/​2307-3497-2023-193-82-100.
Google Scholar

This website uses cookies

We use cookies to enhance your experience and support COUNTER Metrics for transparent reporting of readership statistics. Cookie data is not sold to third parties or used for marketing purposes.

Powered by Scholastica, the modern academic journal management system