The Complete Genome Sequences of 101 Species of Reptiles

Methods

Single wild-collected individuals were sampled for this study from Ethiopia, Guyana, Mexico and the United States. DNA extraction was performed using the Qiagen DNAeasy genomic extraction kit using the standard protocol. A paired-end sequencing library was constructed using the Illumina TruSeq kit according to the manufacturer’s instructions. The library was sequenced on an Illumina Hi-Seq platform in paired-end, 2 × 150 bp format. The resulting fastq files were trimmed of adapter/primer sequence and low-quality regions with Trimmomatic v0.33 (Bolger, Lohse, and Usadel 2014). The trimmed sequence was assembled by SPAdes v2.5 (Bankevich et al. 2012) followed by a finishing step using Zanfona (Kieras, O’Neill, and Pirro 2021).

Results and Data Availability

All data, including raw reads and assembled genome sequence, is available via Genbank. Names in bold indicate material associated with type specimens.

Funding

Funding was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345 Genomic Studies of Eukaryotic Taxa and by NSF grants DBI 2334779 and DEB DEB1501711, awarded to TJC.

Acknowledgements

TJC thanks the Ethiopian Wildlife Conservation Authority, the Guyanese Environmental Protection Agency, SEMARNAT Mexico and the Florida FWC for research, collection and export permits pertaining to fieldwork associated with these specimens.