Methods

Single wild-collected vipers individuals were sampled. DNA extraction was performed using the Qiagen DNAeasy genomic extraction kit using the standard protocol. A paired-end sequencing library was constructed using the Illumina TruSeq kit according to the manufacturer’s instructions. The library was sequenced on an Illumina Hi-Seq platform in paired-end, 2 × 150 bp format. The resulting fastq files were trimmed of adapter/primer sequence and low-quality regions with Trimmomatic v0.33 (Bolger, Lohse, and Usadel 2014). The trimmed sequence was assembled by SPAdes v2.5 (Bankevich et al. 2012) followed by a finishing step using Zanfona (Kieras, O’Neill, and Pirro 2021).

Results and Data Availability

All data is available via Genbank.

taxname NCBI:txid genome_accession
Agkistrodon piscivorus 8715 JBKKFQ000000000
Crotalus aquilus 8743 JBLLKR000000000
Crotalus enyo 125865 JBLLKU000000000
Crotalus mitchellii 384065 JBKKFL000000000
Crotalus viridis 8739 JBKOIX000000000

Funding

Funding was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345 Genomic Studies of Eukaryotic Taxa.