The Complete Genome Sequences of Five Species of Vipers (Viperidae, Squamata)

Abdellah Idrissi Azami; Stacy Pirro; Salsabil Hamdi; Najib Al Idrissi; Noursaid Tligui; Hassan Ghazal

doi:10.56179/001c.129595

Idrissi Azami, Abdellah, Stacy Pirro, Salsabil Hamdi, Najib Al Idrissi, Noursaid Tligui, and Hassan Ghazal. 2025. “The Complete Genome Sequences of Five Species of Vipers (Viperidae, Squamata).” Biodiversity Genomes, February. https://doi.org/10.56179/001c.129595.

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Abstract

We present the complete genome sequences of five species of vipers: Agkistrodon piscivorus, Crotalus aquilus, Crotalus enyo, Crotalus mitchellii, and Crotalus viridis. Illumina sequencing was performed on DNA extracted from wild-caught specimens. The reads were assembled using a de novo method followed by a finishing step. The data is publicly available via Genbank.

Methods

Single wild-collected vipers individuals were sampled. DNA extraction was performed using the Qiagen DNAeasy genomic extraction kit using the standard protocol. A paired-end sequencing library was constructed using the Illumina TruSeq kit according to the manufacturer’s instructions. The library was sequenced on an Illumina Hi-Seq platform in paired-end, 2 × 150 bp format. The resulting fastq files were trimmed of adapter/primer sequence and low-quality regions with Trimmomatic v0.33 (Bolger, Lohse, and Usadel 2014). The trimmed sequence was assembled by SPAdes v2.5 (Bankevich et al. 2012) followed by a finishing step using Zanfona (Kieras, O’Neill, and Pirro 2021).

Results and Data Availability

All data is available via Genbank.

taxname	NCBI:txid	genome_accession
Agkistrodon piscivorus	8715	JBKKFQ000000000
Crotalus aquilus	8743	JBLLKR000000000
Crotalus enyo	125865	JBLLKU000000000
Crotalus mitchellii	384065	JBKKFL000000000
Crotalus viridis	8739	JBKOIX000000000

Funding

Funding was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345 Genomic Studies of Eukaryotic Taxa.

Submitted: February 10, 2025 EDT

Accepted: February 13, 2025 EDT

References

Bankevich, Anton, Sergey Nurk, Dmitry Antipov, Alexey A. Gurevich, Mikhail Dvorkin, Alexander S. Kulikov, Valery M. Lesin, et al. 2012. “SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing.” Journal of Computational Biology 19 (5): 455–77. https://doi.org/10.1089/cmb.2012.0021.

Google Scholar

Bolger, Anthony M., Marc Lohse, and Bjoern Usadel. 2014. “Trimmomatic: A Flexible Trimmer for Illumina Sequence Data.” Bioinformatics 30 (15): 2114–20. https://doi.org/10.1093/bioinformatics/btu170.

Google Scholar

Kieras, M., K. O’Neill, and S. Pirro. 2021. “Zanfona, a Genome Assembly Finishing Tool for Paired-End Illumina Reads.” https://github.com/zanfona734/zanfona.