Methods

Single wild-collected individuals were sampled for this study.

DNA extraction was performed using the Qiagen DNAeasy genomic extraction kit using the standard protocol. A paired-end sequencing library was constructed using the Illumina TruSeq kit according to the manufacturer’s instructions. The library was sequenced on an Illumina Hi-Seq platform in paired-end, 2 × 150 bp format. The resulting fastq files were trimmed of adapter/primer sequence and low-quality regions with Trimmomatic v0.33 (Bolger, Lohse, and Usadel 2014). The trimmed sequence was assembled by SPAdes v2.5 (Bankevich et al. 2012) followed by a finishing step using Zanfona (Kieras, O’Neill, and Pirro 2021).

Results and Data Availability

All data, including raw reads and assembled genome sequence, is available via Genbank.

taxname specimen_voucher accession
Asio madagascariensis FMNH 185845 JBJNIK000000000
Glaucidium cuculoides FMNH 102348 JBEUDJ000000000
Megascops albogularis FMNH 287980 JBIMPI000000000
Megascops choliba FMNH 62510 JBFCBS000000000
Megascops ingens FMNH 287769 JBIMPG000000000
Megascops kennicottii FMNH 74747 JBGOFM000000000
Megascops trichopsis FMNH 137851 JBISCZ000000000
Megascops watsonii FMNH 247145 JBFSXE000000000
Otus brucei FMNH 230012 JBGAFH000000000
Otus elegans FMNH 152192 JBFSXD000000000
Otus icterorhynchus FMNH 270519 JBEUSX000000000
Otus lempiji FMNH 213903 JBEUDB000000000
Otus lettia FMNH 230033 JBFBVQ000000000
Otus longicornis FMNH 429202 JBGOFK000000000
Otus manadensis FMNH 219912 JBGDLS000000000
Otus mirus FMNH 357429 JBFTWZ000000000
Otus nigrorum FMNH 103133 JBGGHY000000000
Otus rutilus FMNH 363793 JBEUDL000000000
Otus scops FMNH 276079 JBDLLQ000000000
Otus semitorques FMNH 226369 JBGDLU000000000
Otus senegalensis FMNH 285590 JBEUDK000000000
Phodilus badius FMNH 229975 JBEALI000000000

Funding

Funding was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345 Genomic Studies of Eukaryotic Taxa and by NSF grant DEB 2203228, awarded to TAC.