Methods

Single wild-collected individuals were sampled for this study.

DNA extraction was performed using the Qiagen DNAeasy genomic extraction kit using the standard protocol. A paired-end sequencing library was constructed using the Illumina TruSeq kit according to the manufacturer’s instructions. The library was sequenced on an Illumina Hi-Seq platform in paired-end, 2 × 150 bp format. The resulting fastq files were trimmed of adapter/primer sequence and low-quality regions with Trimmomatic v0.33 (Bolger, Lohse, and Usadel 2014). The trimmed sequence was assembled by SPAdes v2.5 (Bankevich et al. 2012) followed by a finishing step using Zanfona (Kieras, O’Neill, and Pirro 2021).

Results and Data Availability

All data, including raw reads and assembled genome sequence, is available via Genbank.

taxname specimen_voucher accession
Daptrius ater FMNH 101040 JAVJIE000000000
Falco ardosiaceus FMNH 82377 JAVLTF000000000
Falco berigora KU 134054 JAOPUJ000000000
Falco biarmicus FMNH 250152 JAVJID000000000
Falco cenchroides KU 98805 JAOSZA000000000
Falco cherrug IRGN KBG9ROHKW9 JAOPJX000000000
Falco chicquera FMNH 319931 JAVLTG000000000
Falco columbarius FMNH 364097 JAOPUE000000000
Falco concolor FMNH 100418 JAVJGM000000000
Falco deiroleucus KU 125726 JAOPJS000000000
Falco dickinsoni FMNH 100004 JAVLTL000000000
Falco fasciinucha KU 125719 JAOSLO000000000
Falco femoralis IRGN ZW3NAPDIPV JAOPJR000000000
Falco jugger FMNH 300987 JAVLTJ000000000
Falco moluccensis FMNH 100977 JAUTCE000000000
Falco novaeseelandiae LSUMZ 16740 JAOPUK000000000
Falco pelegrinoides FMNH 369372 JAVCLA000000000
Falco rufigularis LSUMZ 12347 JAOPJT000000000
Falco rupicolus FMNH 101582 JAVJGO000000000
Falco sparverius FMNH 100283 JAVLTI000000000
Falco subbuteo FMNH 474701 JAVKLB000000000
Falco zoniventris FMNH 185836 JAVLTH000000000
Ibycter americanus IRGN 898APBXE3P JAVJGI000000000
Micrastur mirandollei FMNH 250724 JAUZSY000000000
Micrastur ruficollis FMNH 456438 JAVKKW000000000
Microhierax caerulescens FMNH 210986 JAVJGJ000000000
Microhierax latifrons FMNH 211726 JAVJGN000000000
Milvago chimachima FMNH 295956 JAUTDA000000000
Phalcoboenus megalopterus FMNH 101027 JAVJHZ000000000
Polihierax semitorquatus FMNH 369352 JAUTDB000000000

Funding

Funding was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345 Genomic Studies of Eukaryotic Taxa. TAC was supported by NSF grant DEB 2203228.