Methods

Single wild-collected individuals were sampled for this study.

DNA extraction was performed using the Qiagen DNAeasy genomic extraction kit using the standard protocol. A paired-end sequencing library was constructed using the Illumina TruSeq kit according to the manufacturer’s instructions. The library was sequenced on an Illumina Hi-Seq platform in paired-end, 2 × 150 bp format. The resulting fastq files were trimmed of adapter/primer sequence and low-quality regions with Trimmomatic v0.33 (Bolger, Lohse, and Usadel 2014). The trimmed sequence was assembled by SPAdes v2.5 (Bankevich et al. 2012) followed by a finishing step using Zanfona (Kieras, O’Neill, and Pirro 2021).

Results and Data Availability

All data, including raw reads and assembled genome sequence, is available via Genbank.

taxname specimen_voucher accession
Accipiter badius FMNH 447212 JAVHUK000000000
Accipiter francesiae FMNH 393180 JAVHMV000000000
Accipiter madagascariensis FMNH 449187 JAVHUI000000000
Accipiter meyerianus FMNH 153357 JAUTCD000000000
Accipiter minullus FMNH 452457 JAVHUJ000000000
Accipiter rhodogaster FMNH 102059 JAVKKR000000000
Accipiter erythronemius LSUMZ B-38859 JANCMG000000000
Accipiter tachiro FMNH 452456 JAVJIG000000000
Aquila gurneyi FMNH 185069 JAVKKY000000000
Aquila rapax FMNH 293464 JAVKKT000000000
Aviceda cuculoides FMNH 444009 JAVJGG000000000
Aviceda leuphotes FMNH 228511 JAVJGH000000000
Buteo brachyurus FMNH 501306 JAVKKV000000000
Buteogallus lacernulatus LACM Birds-27764 JAQIFQ000000000
Buteogallus meridionalis ANSP 34402 JAOEII000000000
Circaetus cinereus FMNH 285566 JAXCMH000000000
Circaetus pectoralis IRGN W2187 JAVJGK000000000
Circaetus spectabilis FMNH 212981 JAVLTE000000000
Circus macrosceles FMNH 100392 JAUTCC000000000
Clanga clanga FMNH 217891 JAVKKQ000000000
Erythrotriorchis buergersi BBM NG-54096 JAPTGX000000000
Geranoaetus melanoleucus FMNH 296557 JAVKKU000000000
Haliaeetus vocifer FMNH 268827 JAVKKX000000000
Hieraaetus wahlbergi FMNH 474700 JAVJGL000000000
Lophaetus occipitalis FMNH 282642 JAVKKS000000000
Milvus aegyptius FMNH 431139 JAVJIB000000000
Milvus lineatus FMNH 279234 JAVJIC000000000
Morphnarchus princeps ANSP 180156 JAPFFO000000000
Pseudastur albicollis FMNH 456424 JAVKKZ000000000
Pseudastur occidentalis ANSP 183807 JAOYNB000000000
Sagittarius serpentarius IRGN 65UKE4LQWQ JAVKLA000000000

Funding

Funding was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345 Genomic Studies of Eukaryotic Taxa and by NSF grant DEB 2203228, awarded to TAC.