Methods
A single wild-collected specimen was used for sequencing. DNA extraction was performed using the Qiagen DNAeasy genomic extraction kit using the standard process. A paired-end sequencing library was constructed using the Illumina TruSeq kit according to the manufacturer’s instructions. The library was sequenced on an Illumina Hi-Seq platform in paired-end, 2 × 150 bp format. The resulting fastq files were trimmed of adapter/primer sequences and low-quality regions with Trimmomatic v0.33 (Bolger, Lohse, and Usadel 2014). The trimmed sequence was assembled by SPAdes v2.5 (Bankevich, Nurk, Antipov, et al. 2012) followed by a finishing step using Zanfona v1.0 (Kieras 2021) to make additional contig joins based on conserved regions in related species.
Data availability
Raw and assembled data are publicly available via GenBank.
Raw genome data: SRR28478403
Assembled genome: JBCAUF000000000
Funding
Funding was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345.