Methods

Tissues from single, wild-collected individuals were used for this study. DNA extraction was performed using the Qiagen DNEasy genomic extraction kit using the standard process. Paired-end sequencing libraries were constructed using the Illumina TruSeq kit according to the manufacturer’s instructions. The libraries were sequenced on an Illumina Hi-Seq platform in paired-end, 2 × 150bp format. The resulting fastq files were trimmed of adapter/primer sequences and low-quality regions with Trimmomatic v0.33 (Bolger, Lohse, and Usadel 2014). The trimmed sequence was assembled by SPAdes v3.15.4 (Bankevich et al. 2012) followed by a finishing step using Zanfona (Kieras, O’Neill, and Pirro 2021).

Results and Data Availability

All genomes are available on Genbank:
Charaxes varanes JANCMC000000000
Danaus melanippus JAAAKD000000000
Dione vanillae JBCHJV000000000
Doxocopa laurentia JAOPTZ000000000
Dryas iulia JBCHJX000000000
Eueides isabella JBCGHP000000000
Eueides lampeto JBCGHS000000000
Eueides vibilia JBCHJZ000000000
Heliconius antiochus JBCGCW000000000
Heliconius atthis JBDJHX000000000
Heliconius burneyi JBCHJY000000000
Heliconius charithonia JAXGGO000000000
Heliconius clysonymus JBCGHO000000000
Heliconius congener JBCGCV000000000
Heliconius demeter JBCHJW000000000
Heliconius doris JBCGHR000000000
Heliconius egeria JBCGHQ000000000
Heliconius eleuchia JBCGHN000000000
Heliconius elevatus JBCGHU000000000
Heliconius eratosignis JBCHVV000000000
Heliconius hewitsoni JBCHKA000000000
Heliconius leucadia JBCHVU000000000
Heliconius numata JBCHWA000000000
Heliconius pardalinus JBCHVS000000000
Heliconius peruvianus JBCHVZ000000000
Heliconius sapho JBCHVT000000000
Heliconius sara JBCHVQ000000000
Heliconius timareta JBDJHW000000000
Heliconius wallacei JBCHVR000000000
Heliconius xanthocles JBCHVX000000000
Hypolimnas misippus JAOPJQ000000000
Junonia oenone JAPEIZ000000000
Neptis clinioides JAOPUB000000000
Taenaris catops WURW00000000

Funding

Funding was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345 Genomic Studies of Eukaryotic Taxa.