Methods
DNA extraction was performed using the Qiagen DNAeasy genomic extraction kit using the standard protocol. A paired-end sequencing library was constructed for each specimen using the Illumina TruSeq kit according to the manufacturer’s instructions. The libraries were sequenced on an Illumina Hi-Seq platform in paired-end, 2 × 150 bp format. The resulting fastq files were trimmed of adapter/primer sequence and low-quality regions with Trimmomatic v0.33 (Bolger, Lohse, and Usadel 2014). The trimmed sequence was assembled by SPAdes v2.5 (Bankevich et al. 2012) followed by a finishing step using Zanfona (Kieras, O’Neill, and Pirro 2021).
Results and Data Availability
Genome data is available through Genbank:
Specimen information can be found at Spider Parasite Digital Research Collection. Occurrence dataset (ID: 8be51fa1-6dec-44d6-9e3b-a748c7463c45) https://ecdysis.org/collections/misc/collprofiles.php?collid=93.
Funding
Funding for genome sequencing was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345 Genomic Studies of Eukaryotic Taxa. Specimen collection was funded by the Department of Biology at the University of Florida (Research/export permits: RCIV00022018, Authorization 202302008, from the Namibia National Commission on Research, Science, and Technology).