Methods

Tissue from single, wild-collected individuals were used for this study. DNA extraction was performed using the Qiagen DNAeasy genomic extraction kit using the standard process. A paired-end sequencing library was constructed using the Illumina TruSeq kit according to the manufacturer’s instructions. The library was sequenced on an Illumina Hi-Seq platform in paired-end, 2 × 150bp format. The resulting fastq files were trimmed of adapter/primer sequences and low-quality regions with Trimmomatic v0.33 (Bolger, Lohse, and Usadel 2014). The trimmed sequence was assembled by SPAdes v2.5 (Bankevich et al. 2012) followed by a finishing step using Zanfona (Kieras, O’Neill, and Pirro 2021).

Results and Data Availability

All raw read data and assembled genomes are available on Genbank:

Species Raw Read Assembled Genome
Hypoplectrus aberrans SRR19070321 JAOYMT000000000
Hypoplectrus chlorurus SRR18184334 JAOXJR000000000
Hypoplectrus guttavarius SRR18184176 JAOXOB000000000
Hypoplectrus indigo SRR19070349 JAOXJS000000000
Hypoplectrus nigricans SRR18272038 JAOWBK000000000
Hypoplectrus providencianus SRR17839752 JAOWBC000000000
Hypoplectrus randallorum SRR17839729 JAOXNY000000000

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Funding

Funding was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345 Genomic Studies of Eukaryotic Taxa.