Methods

Tissues from single, wild-collected individuals were used for this study.

DNA extraction was performed using the Qiagen DNEasy genomic extraction kit using the standard process. Paired-end sequencing libraries were constructed using the Illumina TruSeq kit according to the manufacturer’s instructions. The libraries were sequenced on an Illumina Hi-Seq platform in paired-end, 2 × 150bp format. The resulting fastq files were trimmed of adapter/primer sequences and low-quality regions with Trimmomatic v0.33 (Bolger, Lohse, and Usadel 2014). The trimmed sequence was assembled by SPAdes v3.15.4 (Bankevich et al. 2012) followed by a finishing step using Zanfona (Kieras, O’Neill, and Pirro 2021).

Results and Data Availability

All raw read data and assembled genomes are available on Genbank:

 
Amissidens hainesi JANZXU000000000
Ariopsis seemanni JAOANJ000000000
Arius gagora JAODTS000000000
Arius madagascariensis JANZXT000000000
Bagre pinnimaculatus JAPEKL000000000
Batrachocephalus mino JAOBAL000000000
Brustiarius nox JAODHU000000000
Brustiarius proximus JAOXJH000000000
Brustiarius solidus JAODKP000000000
Cathorops aguadulce JAPEIY000000000
Cathorops dasycephalus JAPEKN000000000
Cathorops festae JAODXR000000000
Cathorops hypophthalmus JAPEKO000000000
Cathorops melanopus JAODLJ000000000
Cathorops multiradiatus JAPEKM000000000
Cephalocassis borneensis JAOPUC000000000
Cephalocassis melanochir JAODOG000000000
Chinchaysuyoa labiata JAODLF000000000
Cinetodus carinatus JAODOI000000000
Cinetodus crassilabris JAODKQ000000000
Cinetodus froggatti JAODOL000000000
Cochlefelis danielsi JAODKR000000000
Cochlefelis spatula JAODKX000000000
Galeichthys feliceps JAODTV000000000
Genidens genidens JAODOH000000000
Genidens machadoi JAOTJA000000000
Genidens planifrons JAOANL000000000
Hemiarius dioctes JAODID000000000
Hemiarius stormii JAODOD000000000
Nedystoma dayi JAODIC000000000
Nedystoma novaeguineae JAODLB000000000
Nemapteryx armiger JAODOB000000000
Nemapteryx augusta JAODHT000000000
Nemapteryx nenga JAODOC000000000
Neoarius berneyi JAODIF000000000
Neoarius graeffei JALHSM000000000
Neoarius leptaspis JAODTU000000000
Neoarius midgleyi JAPEKJ000000000
Neoarius paucus JAPUBO000000000
Neoarius pectoralis JAPTHB000000000
Neoarius utarus JAODOJ000000000
Netuma bilineata JAODOK000000000
Notarius cookei JAOEHH000000000
Notarius kessleri JAOBAM000000000
Notarius lentiginosus JAODVO000000000
Notarius quadriscutis JANZLB000000000
Occidentarius platypogon JAOBRE000000000
Osteogeneiosus militaris JANZLE000000000
Plicofollis layardi JAODHV000000000
Plicofollis nella JAODOM000000000
Plicofollis tonggol JAODOE000000000
Potamarius nelsoni JAODKY000000000
Potamarius usumacintae JAOEHJ000000000
Potamosilurus coatesi JAODKU000000000
Potamosilurus latirostris JAODKZ000000000
Potamosilurus macrorhynchus JAOBYD000000000
Potamosilurus velutinus JAODLA000000000
Sciades assimilis JAODJS000000000
Sciades dowii JAODKS000000000
Sciades herzbergii JAPFFA000000000
Sciades mastersi JAODKO000000000
Sciades proops JAODOF000000000
Sciades troschelii JAODIB000000000

Funding

Funding was provided by Iridian Genomes grant# IRGEN_RG_2021-1345: Genomic Studies of Eukaryotic Taxa.