Methods

Single wild-collected individuals were used for this study. DNA extraction was performed using the Qiagen DNAeasy genomic extraction kit using the standard protocol. A paired-end sequencing library was constructed for each specimen using the Illumina TruSeq kit according to the manufacturer’s instructions. The libraries were sequenced on an Illumina Hi-Seq platform in paired-end, 2 × 150 bp format. The resulting fastq files were trimmed of adapter/primer sequence and low-quality regions with Trimmomatic v0.33 (Bolger, Lohse, and Usadel 2014). The trimmed sequence was assembled by SPAdes v2.5 (Bankevich et al. 2012) followed by a finishing step using Zanfona (Kieras, O’Neill, and Pirro 2021).

Results and Data Availability

All data is available via Genbank:

 
Agave americana JAVRMC000000000
Agave angustifolia JAXCLS000000000
Agave fourcroydes JAVLRU000000000
Agave karwinskii JAXCLU000000000
Agave potatorum JAVKLE000000000
Agave tequiliana JAVJIF000000000
Annona squamosa JAXCLR000000000
Cedrela odorata JAXCLQ000000000
Pouteria campechiana JAYJMB000000000
Pouteria glomerata JAVXUM000000000
Trichilia hirta JAWDEX000000000
Trichilia minutiflora JAVXUL000000000

Funding

Funding was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345 Genomic Studies of Eukaryotic Taxa and by ECOSUR 5103711808 Fiscal Project for specimen collection and preparation.

Acknowledgments

The authors would like to thank Dr. Eric Edsinger for his participation in the collaboration established between the authors of this work and for his assistance in coordinating the project.